16 InPro 3100 (i) pH electrode
InPro 3100 (i) pH electrode © 08 / 22 METTLER TOLEDO
52 002 293 B Printed in Switzerland
4 Installation and commissioning
1. On unpacking, check the electrode for mechanical
damage of the pH sensitive glass and shaft. Report
any damage immediately to your METTLER TOLEDO
supplier.
2. Removethewateringcapandbrieyrinsetheelectrode
withdeionizedwater.Afterrinsing,theelectrodeshould
onlybedabbeddrywithatissue.DonotrubthepH
sensitive glass, since this can lead to electrostatic
chargingandsluggishresponsetimes.
3. CheckthespacebehindthepHsensitiveglassforthe
presenceofanyairbubblesandremovesamebygently
swingingtheelectrodeinaverticalplane.
4. Please refer to the instruction manual of the relative
housinginordertoinstalltheelectrode.
5. Connect the electrode to the pH transmitter, using a
cable with the appropriate connector (VP connector
forInPro3100).Pleaserefertothewiringandterminal
diagramaccompanyingthecable.
6. Intelligent ISM®electrodessuchasInPro310Xienable
“Plug and Measure” and extended diagnostics. For
installation, commissioning and operation of the
ISM system please observe the instructions for the
transmitter,moduleandcable.
5 Operation
5.1 Calibration of the electrode and pH transmitter
FortheInPro3100(i)electrodea2-pointpHcalibrationis
recommended.Beforecalibration,rstremovethewatering
cap.Tospeeduptheresponsetimeoftheelectrodeafter
longtransportandstorageperiods,andbeforecalibration,
diptheelectrodeintobufferpH7,bufferpH4andbufferpH
7again,eachtimeforaboutoneminute.Theelectrodeis
thendippedsuccessivelyintotwodifferentbuffersolutions
withgivenpHreferencevaluesandthetransmittercalibrated
tothesebuffervalues.NormallyyoustartwithbufferpH7
fordeterminingthe0-pointandthenbufferpH4or9for
determiningtheslope.Inthecaseofmicroprocessor-based
transmitters with buffer recognition, the order of the two
chosenbuffersolutions isirrelevant.Wheneverpossible,
atemperaturesensor(forInPro3100theintegratedone)
should be connected to the transmitter and “automatic
temperaturemeasurement”selectedthere.Pleasereferto
theinstructionmanualofthetransmitterforfurtherdetails.
5.2 Sterilization of electrode
TheInPro3100(i)electrodecanbesterilizedeitherthrough
autoclaving (remove red protection cap) or through in-
situ sterilization with steam or superheated fermentation
medium. Sterilization temperatures above 140°C are not
allowed,andexcessivesterilizationtime(forinstancet>30
minat140°C)could notably shorten the life timeof the
electrode.Repeatedsterilizationand/orautoclavingofthe
electrodecanleadtoaslightcolorationoftheelectrolyte.
This color change does not effect the functioning of the
electrode.
5.3 Temperature sensor of InPro 3100 (analog version)
The integrated Pt100/1000 temperature sensor in the
InPro3100isprimarilyintendedforautomatictemperature
compensationofthepHsignalandnotforanyaccurate
andsafetemperatureindicationorcontroloftheprocess
temperature. If you anyhow want to try this, check that
your transmitter has the required temperature indication
accuracyandafree outletforthesignal.Furthermore,a
calibrationofthetemperaturesignalmightbenecessary.
en